Adenosine-binding to cerebral preparations in interpretation of adenosine activation of adenosine 3':5'-cyclic monophosphate formation [proceedings].

1 1 1 0 20 40 60 Incubation time (min) Incubation time (min) Fig. 1. Metabolism of adenosine (A) and effects of adenosine on (a) total urate synthesis and (b) that de nouo Hepatocytes (3.15 mg dry wt./ml) were suspended in Krebs-Henseleit bicarbonate buffer, containing 5 mM-glucose and 2 m d 14C]formate (0.05 Ci/mol), and incubated at 4OoC in the absence (M, 0) or presence (0,O) of 0.1 mwadenosine. The concentrations of adenosine (A) and urate (I 0)and urate synthesis de nouo ( 0 , O ; equivalent to [l'CIurate formed) were measured as described in the text. The results shown are typical of those obtained in several experiments.